trkb inhibitor ana 12  (MedChemExpress)

Journal: Antioxidants (Basel, Switzerland)

Article Title: A Novel Gastrodin Derivative with Neuroprotection Promotes NGF-Mimic Activity by Targeting INSR and ACTN4 to Activate PI3K/Akt Signaling Pathway in PC12 Cells.

doi: 10.3390/antiox14030344

Figure Lengend Snippet: Figure 3. Screening NGF-mimic activity of GAD037-related potential targets and signaling pathways. (A–I) Effects of inhibitors of TrkA, TrkB, Ras, Raf, ERK, GR, IGF-1R, INSR, PI3K (K252a, ANA-12, FTA, AZ628, U0126, RU486, T9576, HNMPA-(AM)3, and LY294002, respectively) on neurite outgrowth in- duced by GAD037 treatment for 48 h. All experiments with inhibitors were conducted simultaneously in 3rd, 4th, and 9th generations of cells. Data are presented as mean ± SEM. *** p < 0.001 represents significant differences compared with control group; ns indicate no significant differences; # and ### indicate significant differences at p < 0.05 and p < 0.001, respectively.

Article Snippet: The pronase E (CAT No.: HY-114158) was bought from MedChemExpress, Shanghai, China, while tyrosine kinase B (TrkB) inhibitor (ANA-12, CAT No.: S7745) was acquired from Selleck, Shanghai, China.

Techniques: Activity Assay, Protein-Protein interactions, Control

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β -Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells

doi: 10.3390/ijms25189763

Figure Lengend Snippet: β -cyc modulation of the Ras/Raf signaling pathway in PC12 cells. ( A – E ) Effects of inhibitors of TrkA, TrkB, Ras, Raf, ERK (K252a, ANA-12, FTA, AZ628, U0126, respectively) on the neurite outgrowth induced by β -cyc, its combination with NGF, and NGF 1 ng/mL. Each experiment was repeated three times. The data were expressed as a mean ± SEM. *** p < 0.001, compared with the negative control; ## p < 0.01 and ### p < 0.001, compared with the 3 µM β -cyc group; $$ p < 0.01 and $$$ p < 0.001, compared with the combination group of β -cyc with low-dose NGF; & p < 0.05 and && p < 0.01, ns means no significant difference.

Article Snippet: The pronase E (CAT No.: HY-114158) was obtained from MedChemExpress, Shanghai, China, while the TrkB inhibitor (ANA-12, CAT No.: S7745) was obtained from Selleck, Shanghai, China.

Techniques: Negative Control